Skip to content

DNA Sequencing Activity

April 4, 2011

At the beginning of the week, my biology class did an activity in which we sequenced DNA the “old school” way. Down below is the data I collected.

DNA sequence codes for patients:

Norm: ATG GTG CAC CTG ACT CCT GAG GAG AAG TCT GCC

Abby:  ATG GTG CAC CTG ACT CCT GTG GAG AAG TCT GCC

Bob:     ATG GTG CAC CTG ACT CCT GAG GAG TAG TCT GCC

Carol:  ATG GTG CAC CTG ACC CTG AGG AGA AGT CTG CCC

*The dark blue letters in each patience’s sequence show where their sequence is different compared to the normal sequence (Norm).

Protein sequence codes for patients:

Norm: Met Val His Leu Thr Pro Glu Glu Lys Ser Ala

Abby:  Met Val His Leu Thr Pro Val Glu Lys Ser Ala

Bob:     Met Val His Leu Thr Pro Glu Glu Stop

Carol:  Met Val His Leu Thr Leu Arg Arg Ser Leu Pro

*The dark blue letters in each patience’s sequence show where their sequence differs from Norm’s sequence.

In this activity we wrote out DNA and protein sequences for four different patients (#1 Norm, #2 Abby, #3 Bob, #4 Carol). Norm’s sequence was the contorl. The other three patient’s sequences were found and compared to Norm’s in order to tell if they were in a disease state or not. From our data we found that it is possible that every patient besides Norm, could actually be in a disease state. Abby had a point mutation in her sequence which means that only one base changed. Even though only one base changed in her sequence this could still have a big impact on her health because that one base change, changed her protein sequence also. It changed the protein in her seventh codon from Glu to Val which changed it from a positive protein to a hydrophobic protein. A change like that changes the entire shape of the protein and can also have a rather big effect on her health. The third patient, Bob, also only had a one base change in his sequence, but instead of a point mutation he had a truncation mutation. This mutation was huge because it actually caused for protein stoppage at the ninth codon in his sequence. This is a pretty big change in his sequence and most likely also gave him a disease. The last patient, Carol had a huge change in both her DNA and protein sequence. She had a frame shift mutation which basically means that at her fifth codon a C base was inserted instead of a T base which caused for her entire sequence to be shifted over one to the left. This shift also caused for every protein after the fifth codon to be different from the normal protein sequence. Carol’s change was so drastic that I find it very hard to believe that she wouldn’t have some sort of disease.

Note: If you would like you can use the link below to blast the DNA sequences and find out tons of data about them.

BLAST AWAY!!

Advertisements
No comments yet

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s

%d bloggers like this: