DNA Sequencing Activity
At the beginning of the week, my biology class did an activity in which we sequenced DNA the “old school” way. Down below is the data I collected.
DNA sequence codes for patients:
Norm: ATG GTG CAC CTG ACT CCT GAG GAG AAG TCT GCC
Abby: ATG GTG CAC CTG ACT CCT GTG GAG AAG TCT GCC
Bob: ATG GTG CAC CTG ACT CCT GAG GAG TAG TCT GCC
Carol: ATG GTG CAC CTG ACC CTG AGG AGA AGT CTG CCC
*The dark blue letters in each patience’s sequence show where their sequence is different compared to the normal sequence (Norm).
Protein sequence codes for patients:
Norm: Met Val His Leu Thr Pro Glu Glu Lys Ser Ala
Abby: Met Val His Leu Thr Pro Val Glu Lys Ser Ala
Bob: Met Val His Leu Thr Pro Glu Glu Stop
Carol: Met Val His Leu Thr Leu Arg Arg Ser Leu Pro
*The dark blue letters in each patience’s sequence show where their sequence differs from Norm’s sequence.
In this activity we wrote out DNA and protein sequences for four different patients (#1 Norm, #2 Abby, #3 Bob, #4 Carol). Norm’s sequence was the contorl. The other three patient’s sequences were found and compared to Norm’s in order to tell if they were in a disease state or not. From our data we found that it is possible that every patient besides Norm, could actually be in a disease state. Abby had a point mutation in her sequence which means that only one base changed. Even though only one base changed in her sequence this could still have a big impact on her health because that one base change, changed her protein sequence also. It changed the protein in her seventh codon from Glu to Val which changed it from a positive protein to a hydrophobic protein. A change like that changes the entire shape of the protein and can also have a rather big effect on her health. The third patient, Bob, also only had a one base change in his sequence, but instead of a point mutation he had a truncation mutation. This mutation was huge because it actually caused for protein stoppage at the ninth codon in his sequence. This is a pretty big change in his sequence and most likely also gave him a disease. The last patient, Carol had a huge change in both her DNA and protein sequence. She had a frame shift mutation which basically means that at her fifth codon a C base was inserted instead of a T base which caused for her entire sequence to be shifted over one to the left. This shift also caused for every protein after the fifth codon to be different from the normal protein sequence. Carol’s change was so drastic that I find it very hard to believe that she wouldn’t have some sort of disease.
Note: If you would like you can use the link below to blast the DNA sequences and find out tons of data about them.